Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Anti-NFκB Antibody, p65, clone 1G10.2 detects level of NFκB & has been published & validated for use in WB, IF & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NFκB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100)1, 2. NFκB/Rel proteins exist as homo- or heterodimer complexes.
In a majority of unstimulated cells, the NFκB transcription factors are bound to the inhibitory IκB proteins and retained in the cytoplasm in their inactive form. Upon stimulation by multiple inducers, IκBα is rapidly phosphorylated and degraded. This results in the release of the NFκB complex, most commonly the p105/p65 heterodimer. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription.

Immunogen

Epitope: NLS

Synthetic peptide corresponding to the NLS region of human NFκB p65.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.

Format: Purified

Specificity

Other species have not been tested.

Expected to cross-react with bovine based on complete sequence homology.

Detects NFκB p65.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Target description

65 kDa